A b s t r a c t
In this study, trace amounts of aluminum in serum of dialysis patients were chelated with 2-Amino-3- (1H-imidazol-4-yl)propanoic acid (Histidine) and determined by electro-thermal atomic absorption spectrometry (ETAAS). A fast and efficient method based on ionic liquid dispersive liquid-liquid bio-micro- extraction (IL-DLLBME) was developed for the determination of Al cation in human blood serum samples. In this work, a small amount of 1-Hexyl-3-methylimmidazolum hexafluorophosphate ([HMIM] [PF6]) as an extractant solvent was dissolved in acetone as a dispersant solvent and then the binary solution was rapidly injected by a syringe into the serum containing Al3+,Which have already in-vitro chelated by Histidine amino acid (Al-His) at pH = 6.5. After separation, the settled IL-phase was dissolved in ethanol up to 200 μL and 20 μL of samples injected into the ET-AAS by auto-sampler. Various parameters have been studied and optimized for 10 mL of sample. Under the optimum conditions, the enrichment factor (EF), limit of detection (LOD) and working range (peak area mode) were obtained 53, 15 ng L-1 and 0.05-4.1 g L-1 respectively. In vitro Al chelation showed that His can significantly decrease aluminum concentration in serum of dialysis patients. Validation of methodology was confirmed by standard reference material (SRM).
H. Shirkhanloo, H. Z. Mousavi, and M. Mohamadi
RESULTS AND DISCUSSION
In-vitro aluminum chelation from serum is very hard because about 80% of aluminum ions are bound to serum proteins such as albumin and other compounds. However aluminum can be chelated by histidine bio-ligand at pH 6.5 as same as deferoxamine (DFO) in serum of dialysis patients and help us for Al removal from human body. In this study, we used IL-DLLBME method based on histidine bio-ligand for micro-extraction and determination of aluminum in water and human biological samples.