_1574512518 1574512518 Hamid Shirkhanloo sahar.zi67@gmail.com Hamid Shirkhanloo Analytical Methods in Environmental Chemistry Journal AMECJournal 2645-5382 2645-5552 09 25 2019 2 03 10.24200/amecj.v2.i03 http://journal.amecj.com/index.php/AMECJ-01/issue/view/9 *Negar Motakef Kazemi In this research, the metal–organic framework (MOF) as a solid phase was used for separation mercury [Hg (II)] inhuman serum sample by ultrasound assisted- Ionic Liquid-solid phase microextraction procedure (USA- IL-μ-SPE). Mercury extracted from serum sample by [Zn2(BDC)2(DABCO)]n as MOF at pH=7.8. Hydrophobic ionic liquid ([BMIM] [PF6]) was used as solvent trap for Hg-MOF-NC from the sample solution. The phase of Hg-MOF-NC was back extracted by 0.5 mL of HNO3 (0.2 mol L-1) and finally mercury concentration determined with cold vapor-atomic absorption spectrometry (CV-AAS) after dilution with 0.5 mL of DW. Under the optimal conditions, the linear range, limit of detection and preconcentration factor were obtained 0.02–5.5 µg L−1, 6.5 ng L−1 and 9.8 for serum samples, respectively (%RSD<5%). The validation of methodology was confirmed by standard reference materials (SRM). 11 13 2019 67 78 http://creativecommons.org/licenses/by/4.0 10.24200/amecj.v2.i03.68 http://journal.amecj.com/index.php/AMECJ-01/article/view/68 http://journal.amecj.com/index.php/AMECJ-01/article/download/68/122 http://journal.amecj.com/index.php/AMECJ-01/article/download/68/122 http://journal.amecj.com/index.php/AMECJ-01/article/download/68/132