_1546773116 1546773116 Hamid Shirkhanloo sahar.zi67@gmail.com Hamid Shirkhanloo Analytical Methods in Environmental Chemistry Journal AMECJournal 2645-5382 0000-0000 12 01 2018 1 01 Hamid Shirkhanloo Sara Davari Farnaz Hosseini The ultrasound assisted- dispersive solid phase microextraction method (USA-SPME) was used for in-vitro study on separation/extraction of calcium ions in human blood of chronic kidney disease (CKD). In this procedure, amine-functionalized bimodal mesoporous silica nanoparticle (NH2-UVM7) as a solid phase was used for in-vitro separation/extraction of calcium from blood/serum samples. Moreover, a mixture of NH2-UVM7 with ionic liquid and acetone (S/IL/Ac) was added to serum/blood sample containing of Ca (II) at pH of 7.3. After ultrasonic bath and centrifuging, NH2-UVM7/ IL settled down in bottom of tube, which was extracted Ca (II) ions by binding to amine group ([Ca]2+ →: NH2 ─ UVM7). The concentration of Ca (II) was determined by flame atomic absorption spectrometry (F-AAS, N2O, C2H2) after back extraction remained adsorbent in IL by 0.5 mL of HNO3 (0.5 M). 12 12 2018 57 66 10.24200/amecj.v1.i01.37 http://journal.amecj.com/index.php/AMECJ-01/article/view/37 http://journal.amecj.com/index.php/AMECJ-01/article/download/37/41 http://journal.amecj.com/index.php/AMECJ-01/article/download/37/41 http://journal.amecj.com/index.php/AMECJ-01/article/download/37/43 http://journal.amecj.com/index.php/AMECJ-01/article/download/37/42