Measurement of heavy metals in soil, plants and water samples based on MWCNTs modified with Bis(triethoxysilylpropyl)tetrasulfide by flame atomic absorption spectrophotometry

Volume 5, Issue 01, Pages 49-60, Mar 2022 *** Field:Analytical Method in Environment samples

  • Mohammad Reza Rezaei Kahkha Department of Health Engineering, Zabol University of Medical Sciences, Zabol, Iran
  • Ahmad Salarifar, Corresponding Author, Environmental Engineering Department, Faculty of Natural Resources, Islamic Azad University, Bandar Abbas Branch, Iran
  • Batool Rezaei Kahkha Department of Health Engineering, Zabol University of Medical Sciences, Zabol, Iran
Keywords: Heavy Metals, Environment sample, Bis(triethoxysilylpropyl)tetrasulfide, Uniform dispersive -micro-solid phase extraction, Flame atomic absorption spectrophotometer


Heavy metals (HMs) are considered as the major environmental pollutants that accumulated in soil and plant. Consumption of such contaminated plants by humans and animals would ultimately harm the health of communities. This study aims to evaluate the amount of copper(Co), cadmium(Cd), and lead(Pb) in soil and cultivated plants that are irrigated by the city of Zabol’s wastewater. Also, the heavy metals determined in 20 mL of Zabol’s water based on Bis(triethoxysilylpropyl)tetrasulfide (S4[C3H6Si(OEt)3]2, TEOSiP-TS) modified on MWCNTs as an adsorbent by the uniform dispersive -micro-solid phase extraction (UD-µ-SPE) at optimized pH. In this study, 52 samples including wheat, corn grain, and wild spinach, as well as agricultural soil were selected randomly from three village stations. The concentrations of heavy metals in plants, soils, and water samples were measured using a flame atomic absorption spectrometer (F-AAS).  By optimizing parameters, the linear range (LR) and the detection limit (LOD) of Cu, Cd, and Pb were obtained 1.5-1000 μg L-1, 1-200 μg L-1, 5-1500 μg L-1 and 0.5 μg L-1, 0.25 μg L-1, 1.5 μg L-1, respectively in water samples (RSD%<2). This study indicates that irrigation of agricultural fields using wastewater causes the accumulation of heavy metals in soil and plants.


S. Qayyum, I. Khan, K. Meng, Y. Zhao, C. Peng, A review on remediation technologies for heavy metals contaminated soil, Cent. Asian J. Environ. Sci. Technol. Innov., 1 (2020) 21-29.

.R.R. Kahkha, S. Bagheri, R. Noori, J. Piri, S. Javan, Examining total concentration and sequential extraction of heavy metals in agricultural soil and wheat, Polish J. Environ. Studies, 26 (2017) 2021-2028.

B. He, W. Wang, R. Geng, Z. Ding, D. Luo, J. Qiu, G. Zheng, Q. Fan, Exploring the fate of heavy metals from mining and smelting activities in soil-crop system in Baiyin, NW China, Ecotoxicol. Environ. Safe., 207 (2021) 111234.

F. Elbehiry, H. Elbasiouny, R. Ali, E.C. Brevik, Enhanced immobilization and phytoremediation of heavy metals in landfill contaminated soils, Water Air Soil Pollut., 231 (2020) 1-20.

T. Houri, Y. Khairallah, A. Al Zahab, B. Osta, D. Romanos, G. Haddad, Heavy metals accumulation effects on the photosynthetic performance of geophytes in Mediterranean reserve, J. King. Saud. Uni. Sci., 32 (2020) 874-880. .

T.M. Minkina, S.S. Mandzhieva, M.V. Burachevskaya, T.V. Bauer, S.N. Sushkova, Method of determining loosely bound compounds of heavy metals in the soil, Methods X, 5 (2018) 217-226.

F. Capitelli, F. Colao, M. Provenzano, R. Fantoni, G. Brunetti, N. Senesi, Determination of heavy metals in soils by laser induced breakdown spectroscopy, Geoderma, 106 (2002) 45-62.

H.L. Byers, L.J. McHenry, T.J. Grundl, XRF techniques to quantify heavy metals in vegetables at low detection limits, Food Chem. X, 1 (2019) 100001.

S. Li, C. Zhang, S. Wang, Q. Liu, H. Feng, X. Ma, J. Guo, Electrochemical microfluidics techniques for heavy metal ion detection, Analyst, 143 (2018) 4230-4246.

A. Maurya, L. Kesharwani, M.K. Mishra, Analysis of heavy metal in soil through atomic absorption spectroscopy for forensic consideration, Int. J. Res. Appl. Sci. Eng. Technol., 6 (2018) 1188-1192.

G. Yu, Y. Lu, J. Guo, M. Patel, A. Bafana, X. Wang, B. Qiu, C. Jeffryes, S. Wei, Z. Guo, Carbon nanotubes, graphene, and their derivatives for heavy metal removal, Adv. Compos. Hybrid. Mater., 1 (2018) 56–78.

M. hirani, A. Semnani, S. Habibollahi, H. Haddadi, Ultrasound-assisted, ionic liquid-linked, dual-magnetic multiwall carbon nanotube microextraction combined wif electrothermal atomic absorption spectrometry for simultaneous determination of cadmium and arsenic in food samples, J. Anal. At. Spectrom., 30 (2015)1057–1063.

A.A. Mamun, Y.M. Ahmed, M.F.R. AlKhatib, A.T. Jameel, M. AlSaadi, Lead sorption by carbon nanofibers grown on powdered activated carbon—kinetics and equilibrium, NANO: Brief Reports Rev., 10 (2015) 1550017.

M.F. Mahmoud, N.A. Fekry, M.M.A. El-Latif, Nanocomposites of nanosilica-immobilized-nanopolyaniline and crosslinked nanopolyaniline for removal of heavy metals, Chem. Eng. J., 304 (2016) 679–691.

D.C. Culita, C.M. Simonescu, R.E. Patescu, M. Dragne, N. Stanica, O. Oprea, o-Vanillin functionalized mesoporous silica coated magnetite nanoparticles for efficient removal of Pb(II) from water, J. Solid State Chem., 238 (2016) 311–320.

P. Pirkwieser, J.A. López-López, W. Kandioller, B.K. Keppler, C. Moreno, F. Jirsa, Novel 3-hydroxy-2-naphthoate-based task-specific ionic liquids for an efficient extraction of heavy metals, Front. Chem., 6 (2018)172.

M. Hossein Habibollahi, Extraction and determination of heavy metals in soil and vegetables irrigated wif treated municipal wastewater using new mode of dispersive liquid-liquid microextraction based on teh solidified deep eutectic solvent followed by GFAAS, J. Sci. Food Agric., 30 (2019) 656-665.

M.G. Kakavandi, M. Behbahani, F. Omidi, G. Hesam, Application of ultrasonic assisted-dispersive solid phase extraction based on ion-imprinted polymer nanoparticles for preconcentration and trace determination of lead ions in food and water samples, Food Anal. Methods, 10 (2017) 2454–2466.

M. D. Yadav, K. Dasgupta, Role of sulfur source on the structure of carbon nanotube cotton synthesized by floating catalyst chemical vapour deposition, Chem. Phys. Lett., 748 (2020)137391.

V. Kumar, A. Sharma, P. Kaur, G.P.S. Sidhu, A.S. Bali, R. Bhardwaj, A.K. Thukral, A. Cerda, Pollution assessment of heavy metals in soils of India and ecological risk assessment: A state-of-the-art, Chemosphere, 216 (2019) 449-462.

B. Jiang, A. Adebayo, J. Jia, Y. Xing, S. Deng, L. Guo, Y. Liang, D. Zhang, Impacts of heavy metals and soil properties at a Nigerian e-waste site on soil microbial community, J. Hazard. Mater., 362 (2019) 187-195.

M. Sulaiman, K. Salawu, A. Barambu, Assessment of concentrations and ecological risk of heavy metals at resident and remediated soils of uncontrolled mining site at Dareta Village, Zamfara, Nigeria, J. Appl. Sci. Environ. Manage., 23 (2019) 187–193.

How to Cite
Rezaei Kahkha, M., Salarifar, A., & Rezaei Kahkha, B. (2022). Measurement of heavy metals in soil, plants and water samples based on MWCNTs modified with Bis(triethoxysilylpropyl)tetrasulfide by flame atomic absorption spectrophotometry. Analytical Methods in Environmental Chemistry Journal, 5(01), 49-60.
Original Article