Determination of manganese in rat blood samples based on ionic liquid-liquid extraction and chelation therapy for evaluation of manganese toxicity in rats

Volume 4, Issue 04, Pages 78-91, Dec 2021 *** Field: Medical Chemistry

  • Seyed Jamilaldin Fatemi, (Corresponding Author) Department of Chemistry, Shahid Bahonar University of Kerman, 76169- 133, Kerman, Iran
  • Tayyebeh Zandevakili Department of Chemistry, Shahid Bahonar University of Kerman, Kerman, Iran
  • Fatemeh Khajoee Nejad Department of Chemistry, Shahid Bahonar University of Kerman, Kerman, Iran
  • Marziyeh Iranmanesh Department of Chemistry, Kerman Branch, Islamic Azad University, Kerman, Iran
  • Mohammad Faghihi Zarandi Department of Foreign Languages, Shahid Bahonar University of Kerman, Kerman, Iran
Keywords: Manganese, Chelators, Red blood cells, Toxicity, , Ionic liquid-liquid extraction, Flame atomic absorption spectrometry


In this study, manganese was extracted in the blood of rats based on desferrioxamine (DFO), deferasirox (DFX) and deferiprone (DFP) as chelators (ligands) by ionic liquid-liquid phase extraction method (ILLEM) before being determined by F-AAS. Also, the toxic effects of manganese on blood serum and hematology parameters such as RBC, WBC, HGB, PLT and HCT were investigated. Male Wistar rats were randomly divided into control and toxic groups. Manganese chloride was administrated orally in low and high doses. Orally (deferasirox and deferiprone) or intraperitoneally (desferrioxamine) for 2 weeks. Results showed that exposure to manganese significantly increased both counts of hematology parameters and concentration of this metal ion in serum compared to the control group. First, manganese was chelated with ligands and then the hydrophobic ionic liquid (IL, [HMIM][PF6]) was added to rat blood samples, after shaking and centrifuging, the upper liquid phase was separated by auto-sampler and mixture of IL/ligand in the bottom of conical tube back-extracted and determined with F-AAS. The linear range, LOD and enrichment factor for 10 mL of blood rats were obtained 25- 180 μg L-1, 6.5 μg L-1 and 19.92, respectively.  By chelation therapy manganese was removed from serum and hematology parameters were normal level.


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How to Cite
Fatemi, S. J., Zandevakili, T., Khajoee Nejad, F., Iranmanesh, M., & Faghihi Zarandi, M. (2021). Determination of manganese in rat blood samples based on ionic liquid-liquid extraction and chelation therapy for evaluation of manganese toxicity in rats. Analytical Methods in Environmental Chemistry Journal, 4(04), 78-91.
Original Article