Simultaneously determination of copper and zinc in human serum and urine samples based on amoxicillin drug by dispersive ionic liquid- liquid microextraction coupled to flame atomic absorption spectrometry

Volume 3, Issue 03, Pages 32-43, Sep 2020 *** Field: Pharmaceutical Analytical Chemistry

  • Kian Azami, (Corresponding Author)* Department of Pharmacology and Toxicology, Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, Iran
  • Seyed Mojtaba Mostafavi Department of Chemistry, Iranian-Australian Community of Science, Hobart, Tasmania, Australia
Keywords: Amoxicillin drug, Copper and zinc, Serum and urine samples, Dispersive ionic liquid cloud point extraction procedure, Flame atomic absorption spectrometry


In this work the effect of amoxicillin on copper and zinc (Cu and Zn) deficiency was evaluated by determining of Cu and Zn concentration in human serum and urine samples. By dispersive ionic liquid cloud point extraction procedure (DIL-CPE), 0.03 g of pure amoxicillin drug was added to mixture of 0.1 g of hydrophobic ionic liquid and 0.2 mL acetone which was injected to 2 mL of serum or urine samples which was diluted with DW up to 10 mL. The cloudy solution was shacked for 7 min and Cu and Zn ions was extracted based on sulfur group on amoxicillin ligand at pH of 7 by DIL-CPE. Then, the solution centrifuged and after back extraction with I mL of nitric acid (0.2 M), the remained solution was determined by flame atomic absorption spectrometry (F-AAS).


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How to Cite
Azami, K., & Mostafavi, S. M. (2020). Simultaneously determination of copper and zinc in human serum and urine samples based on amoxicillin drug by dispersive ionic liquid- liquid microextraction coupled to flame atomic absorption spectrometry. Analytical Methods in Environmental Chemistry Journal, 3(03), 32-43.
Original Article